Journal: Frontiers in Immunology

Article Title: Shared and Unique Features Distinguishing Follicular T Helper and Regulatory Cells of Peripheral Lymph Node and Peyer’s Patches

doi: 10.3389/fimmu.2018.00714

Figure Lengend Snippet: Further genes of interest. (A,B) Microarray data for mRNA expression levels of the depicted genes. Same data source as in Figure E. (C) Gating strategy illustrating definition of memory B cells in Peyer’s Patches (PP). (D) Data summary showing frequencies of memory B cells in PP of Sostdc1 +/+ and Sostdc1 −/− mice. Each dot represents one mouse. Shown are mean ± SD. (E) Determination of fecal IgA by ELISA, samples were tested in duplicates. Mean ± SD are given for each dilution step. (F) Left panel: Frequencies of PD1 hi follicular T cells among all CD4 + cells in PP of Sostdc1 +/+ and Sostdc1 −/− mice according to the gating criteria shown in Figure A. Right panel: Frequencies of TFR cells among follicular cells of PP. Foxp3 was detected by intracellular stain as described in Section “ .” Shown are mean ± SD. One-way ANOVA followed by Tukey’s post hoc analysis was performed in (A,B) Data from three independent experiments ( n = 7) were pooled and two-tailed t -tests were done in (D–F) . N/A: not applicable, ns: not significant ( p > 0.05), * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: B6.Cg-Foxp3 (Foxp3) mice were provided by B. Malissen (Aix-Marseille Université, Marseille, France) and B6.Sostdc1 tm1(KOMP)Vlcg (Sostdc1 −/− ) mice by G. Loots (Lawrence Livermore National Laboratory, Livermore, USA).

Techniques: Microarray, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test

Journal:

Article Title: Intracellular Helicobacter pylori in gastric epithelial progenitors

doi: 10.1073/pnas.0407657102

Figure Lengend Snippet: Distribution of H. pylori in gnotobiotic tox176 mice. (A and B) Multilabel immunohistochemical study of stomachs from 7-week-old germ-free normal (A) and tox176 (B) mice showing that tox176-mediated parietal cell ablation results in amplification of mitotically active GEPs. Animals were treated with BrdUrd (green) 90 min before death to label cells in S-phase. Dolichos biflorus agglutinin (red) is used to mark parietal cells. Pit cells are tagged with Alexa Fluor 350-conjugated Anguilla anguilla agglutinin (blue), and neck cells are tagged with Alexa Fluor 350- and Alexa Fluor 647-tagged Griffonia simplicifolia II lectin (purple). GEPs produce NeuAcα2,3Galβ1,4 glycans (white after staining with biotinylated MAA and Cy3-labeled streptavidin). (C) Attachment of CAG7:8 (red) to NeuAcα2,3Galβ1,4-positive GEPs (marked green with MAA) in a stomach from a 15-week-old gnotobiotic tox176 mouse killed after 4 weeks of infection. Nuclei are stained blue with bis-benzimide. (D) Frame from a 3D confocal microscopic projection of a focal area of infection in a tox176 stomach showing intracellular bacteria (red, arrows). Cell borders are delineated by using an antibody to E-cadherin (green after treatment with Alexa Fluor 488-tagged donkey anti-rat Ig). To view the 3D projection in its entirety, see Movie 1, which is published as supporting information on the PNAS web site. (Bars, 10 μm.)

Article Snippet: The following lectins, obtained from EY Laboratories, were used at a final concentration of 20 μg/ml blocking buffer: ( i ) Maackia amurensis agglutinin (MAA) (detects GEPs expressing NeuAcα2,3Galβ1,4-containing glycans); ( ii ) Dolicos biflorus agglutinin (specific for parietal cells in the FVB/N strain); ( iii ) Anguilla anguilla agglutinin (pit cells); or ( iv ) Griffonia simplifolica II (neck cells).

Techniques: Immunohistochemical staining, Amplification, Staining, Labeling, Infection